The application of the single-chamber piezoelectric pump is limited in many fields,for it has self-priming performance and bubble tolerance. It is an efficient way to adopt a multi-chamber structure to enhance the performance of piezoelectric pumps. The analysis result on changing processes of the volume and pressure in the chamber of a pump shows that the double-chamber serial piezoelectric pump only can be driven by a tandem mode,and the double-chamber parallel piezoelectric pump only can be driven by a parallel mode,respectively. The experiment results indicate that the maximum flow of the double-chamber serial pump is 1150 ml/min in a sine wave voltage of 200 V and a frequency of 152 Hz,and that of double-chamber parallel pump is 640 ml/min in a sine wave voltage of 140 V and a frequency of 220 Hz.These data reported here show that the multi-chamber with a serial structure can enhance the efficiency and performance of pumps.