To address the challenges of difficult fingerprint extraction in situ and the risk of damaging fingerprints from improper handling, this study proposes a new method using an aggregation-induced emission (AIE) reagent (C₂₇H₁₉N₃SO) combination with a microgrid atomization technique based on the time-resolved imaging principles. This study analyzes the imaging effectiveness and influence of background colors on fingerprint fluorescence extraction. The extraction quality is evaluated by analyzing the gray values and the number of identified secondary fingerprint features. Experimental results indicate that the AIE reagent in situ aggregation-induced activation offers remarkable advantages for latent fingerprint imaging. Unlike traditional chemical reagent development methods, proposed method improves the contrast between fingerprints and their backgrounds, making it both efficient and nondestructive. The proposed method can reliably extract over 100 consecutive oily latent fingerprints. In addition, this study defines the applicable scope of the AIE reagent, enriches the methodological framework for latent fingerprint development, and holds practical value with promising potential for widespread application.