Carbon Fiber Reinforced Plastics (CFRP) are lightweight and high strength composites that are widely used in aerospace and other fields. The 2D-T800 carbon fiber reinforced composites were prepared based on Vacuum Impregnation Hot-Pressing System (VIHPS), and 5 000 cyclic loading experiments were carried out under the conditions of 500 MPa load, 15 Hz sinusoidal loading waveform, and stress ratio of 0.1, and the mechanical behaviors of the composites with Graphene Oxide (GO) mass fraction (w(GO)) of 0%, 0.25%, 0.50%, 0.75%, and 1.00% respectively were investigated. The microstructures were characterized by scanning electron microscopy, and the fracture morphology was observed to investigate the effect of GO content on the fatigue tensile properties of the composites. The results showed that the fatigue tensile strength of CFRP showed a tendency of increasing and then decreasing with the increase of GO content, and the optimum fatigue tensile performance was achieved when the content of GO was 0.50%, with the fatigue tensile strength of 1 123.34 MPa and the elastic modulus of 77.92 GPa. The fatigue tensile strength was reduced by 25.39% when the w(GO) was 1.00%. When the w(GO) was 0.50%, GO was uniformly coated on the carbon fiber filaments in a scale-like manner, which increased the stress transfer pathway, reduced the porosity, and the matrix was sufficiently infiltrated, thus significantly improving the fatigue tensile strength of CFRP. When the w(GO) is increased to 1.00%, significant agglomeration of high content of GO occurs on carbon fiber filaments, which leads to a serious impact on the matrix infiltration effect and a decrease in fatigue strength.