Experimental investigation of Talbot self-imaging effect of an amplitude grating under illumination of femtosecond laser pulse -- the FemtoTalbot effect is reported. Theoretical analyzed results show that Talbot images under illumination of femtosecond laser pulses are not the same as that under continuous wave illumination. Experimental results are in good agreement with the theoretical analysis. We believe that the experimental investigation of the FemtoTalbot effect is highly interesting for the enormous potential applications of Talbot effect.