Materials with large two-photon absorption (TPA) cross sections have the potential applications such as optical power limiting. Herein, the optical limiting of a series of multibranched chromophores based on triphenylamine is reported, and the comparative study of their two-photon absorption property pumped by 800 nm nanosecond and femtosecond laser pulses, associative with the fluorescence lifetime on the one-photon excitation, is performed. Results show that, with higher generation, multibranched molecules possess longer excited lifetime and stronger re-absorption on the excited state. Thus, the effective two-photon absorption cross-section pumped by nanosecond laser pulse is larger. As a result, larger optical limiting efficiency is obtained.