In order to improve the performance of the He-Ne laser in the laser feedback measurement system, and solve the technical problem that the frequency cannot be stabilized by traditional methods when the laser feedback mirror is constantly moving, a closed-loop passive frequency stabilization system method based on temperature feedback was adopted to control the temperature of the laser tube, and the theoretical analysis and experimental verification was conducted. The stability of the system under different stabilization temperature and ambient temperature difference was studied. The experimental results show that the best temperature difference of the system is 25.6℃. After frequency stabilization under this temperature difference, the He-Ne laser’s wavelength fluctuation range is 10-4, that is, the frequency stability reaches 1.61×10-7, and power drift is less that 3.20%. The system can adjust the frequency stabilization temperature point according to the change of the ambient temperature, and the frequency stabilization structure is simple, meet the requirements of laser feedback for general application system stability.