Tissue optical clearing provides a good solution to some problems, such as biological tissue has high scattering and turbidity, which seriously affects the contrast and resolution of biological tissue imaging. Ex vivo porcine skin surfaces are coated with OCA such as polyethylene glycol, glycerol and propanediol in different concentrations. Use OCT to dynamically monitor it, and effectively extract the measured signal as it changes with the measured depth during penetration. The results show that the optical transparency technology can increase the imaging depth of OCT. However, for different OCA, the effect of different depth is different. This study provides a valuable data reference for the optimal choice of OCA concentration and time for different depths. This study gives us a further understanding of the mechanism of OCA and will be helpful in guiding the use of OCA in medicine.