In order to investigate the effects of the combined application of water-soluble fertilizer containing humic acid and pesticide on the control effect of flue-cured tobacco bacterial wilt and agronomic traits, a field plot experiment is carried out. Seven treatments are set in the experiment. The treatment without the application of water-soluble fertilizer containing humic acid and pesticide is used as the control (CK). Treatments T1, T3 and T5 are applied with 15.0, 22.5 and 30.0 L/hm² of water-soluble fertilizer containing humic acid, respectively, and combined application of 63 g/hm² of the active ingredient of 12% prothioconazole-kasugamycin suspension. And treatments T2, T4 and T6 are applied with 15.0, 22.5 and 30.0 L/hm² of water-soluble fertilizer containing humic acid, respectively, and combined application of 281 g/hm² of the active ingredient of 37.5% copper hydroxide suspension. The results show that treatments T1 to T6 all can improve the agronomic traits of flue-cured tobacco such as plant height, stem circumference, fresh leaf weight, and leaf expansion degree. The combined application of humic acid and pesticide has a synergistic effect on the control of bacterial wilt. Among them, the control effect of treatment T5 on bacterial wilt is significantly higher than that of other treatments. The control effect of 12% prothioconazole-kasugamycin suspension on bacterial wilt is better than that of 37.5% copper hydroxide suspension. The combined application of humic acid and pesticide has a significant effect on improving the agronomic traits of flue-cured tobacco and controlling bacterial wilt, providing a new way for the comprehensive control of flue-cured tobacco bacterial wilt.