To study the inhibitory effect of red/near-infrared light irradiation therapy (LED phototherapy) on pain, the expression of pain related inflammatory factors and chemokines were analyzed in peripheral blood monocytes. Base on evaluating the safe dose of red light/infrared light with human monocyte THP-1, PBMC from peripheral blood monocytes of healthy adults were prepared. After red light/infrared light irradiation, the expression of pain related inflammatory factors and chemokines were detected by fluorescence quantitative PCR. After treatment with red/infrared light, the gene expression levels of CXCL12, CXCL13, CX3Cl1, CCl4, CCR2, IL-4, IL-6 and MMP2 in PBMC in the LED group were significantly down regulated compared with the control group. Red/near infrared light phototherapy can play an antipain role by inhibiting the expression of pain related chemokines and inflammatory factors in PBMC. This study may provide new ideas for pain adjuvant treatment.