The heat conduction in frozen biological tissue irradiated by high - power short - pulse laser was investigated experimentally. The micro - scale film resistance thermometer with 1 JAS response time was used to measure temperature. The output of the film resistance thermometer was recorded dynamically by a YOKOGAWA DL2700 digital oscilloscope. The experimental results showed that, when the pulse duration is short, the heat conduction in frozen biological tissue is of non - Fourier behavior. The shorter the pulse duration and the thinner the biological tissue, the more notable the non -Fourier heat conduction. The power density and spot size will influence the maximum value of temperature during laser heating, and have nothing t& do with the problem whether or not the non - Fourier heat conduction can take place.