Objective:To obtain a new therapy of port-wine stain (PWS) with good effect and few side effects. Methods: the Leghorn combs chosen as the model and treated with small dose HpD and variable pulsed 532nm laser were evaluated clinically using four different scoring systems commonly used in previous publication. Results: It was shown significant difference among 5 groups (p<0. 01) , and the treatment groups have better effect than the control group. There's also difference among the four treatment groups and the higher dose of HpD has better effect. In all groups the higher the fluency of laser was usedm, the better the effect produced. Conclusion: The results provide evidence that small dose HpD and variable pulsed 532nm laser can increase the efficacy of treatment in PWS. Increasing the fluency of laser and the dose of HpD can improve the efficacy.