Edible Fungi of China, Volume. 44, Issue 3, 53(2025)
Screening of Formulas for Stock Culture Subculture and Liquid Culture of Ganoderma leucocontextum
In order to screen suitable culture medium formula for subculture and liquid culture of Ganoderma leucocontextum, the experiment tested the effects of mycelial growth about selecting different media during the subculturing of the culture and adding different corn processed products to the liquid mediumon. At the same time, the fruiting experiment of the liquid spawn was carried out. The results indicated that changing the suitable culture medium during subculturing of the culture of G. leucocontextum is beneficial for the growth of mycelium, and the growth rate of mycelia was faster when the amount of nitrogen source was reduced appropriately. In liquid culture, adding different corn processed products had different effects on the growth of mycelial pellet. Among them, the cultivation effect of directly adding 8 g·L-1 corn flour (formula Y6) was the best. Under the conditions of this formula, the dry mass of the mycelial pellets was the largest, the quantity was the greatest, and the diameter was the smallest. The fruiting test also showed that the performance of fruit bodies of the liquid spawn with formula Y6 was better.
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CHEN Dongmei, LI Yifan, CHEN Duoyang, LI Gang, HU Huiping, ZHANG Zhi. Screening of Formulas for Stock Culture Subculture and Liquid Culture of Ganoderma leucocontextum[J]. Edible Fungi of China, 2025, 44(3): 53
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Received: Jan. 9, 2025
Accepted: Aug. 26, 2025
Published Online: Aug. 26, 2025
The Author Email: ZHANG Zhi (736737574@qq.com)