A 1940 nm and 980 nm laser pain stimulus system is built to explore the effect of baseline temperature control, oxyhemoglobin solution and laser irradiation area control on laser pain stimulus. With pig skin, a thermocouple module is used to monitor the change of the skin temperature under baseline temperature control and 20% oxyhemoglobin solution; the shape and size of the light is controlled with the help of filters. By using the 1940 nm laser control of baseline temperature of 40 ℃ is realized and joint pain stimulus experiment is carried out with the help of the 980 nm laser. Surface temperature drop time under the condition of 20% oxyhemoglobin solution (110 ms) is much lower than the blank control (1.2 s) and air cooling heat dissipation (341 ms), proving that this is an ideal method to improve pain signal quality. Temperature field distribution with three different filters is listed, proving that the shape and size of the pain stimulus is controllable. The three technologies in the dual-wavelength pain stimulus mode mentioned above can improve the result of the laser pain stimulus and prove the feasibility and credibility of this system.