The determination of chemical oarcinogen-DNA adducts is necessary for research of molecular epidemiology and mechanism of chemical carcinogenesis. A method ig presented in this paper for the detection of putative addnots by using fluorescence spectroscopy. The main advantages include high sensitivity and high selectivity. The minimum detectable amount of O6-MEG is 0.01 fmol/ml. The sensitivity of detection is an order of magnitude higher than that of radioimmunoassay.