Using laser cladding technology to prepare nickel-based tungsten carbide reinforced coating on the surface of 42CrMo steel substrate, this paper studied the microstructure, element composition, phase composition, microhardness and wear resistance of the nickel-based tungsten carbide reinforced coating under three different binders that were prepared by adding rosin alcohol solution, a mixed solution of cellulose acetate and acetone, and an aqueous solution of water glass. Results show that the phase composition of the laser cladding coating is γ-Ni(Fe), Ni3Fe, WC, Cr23C6, Cr7C3. When the rosin alcohol solution is used as the binder, the bottom of the coating obtained is cell crystals and some dendritic dendrites. Moreover, the structure distribution is dense and uniform, and the middle of the coating is mostly dendritic dendrites. The average hardness is 739.4 HV, and the average friction coefficient is 0.53. The average wear volume is 14.07×10-3 mm3, which are reduced by about 13% and 68.5%, respectively, compared to the matrix. The form of wear is slight abrasive wear and fatigue wear, which improves the wear resistance of the coating.