In order to distinguish different kinds of pollen, Pinus massoniana Lamb pollen and seven kinds of bee pollen (camellia pollen, lotus pollen, rose pollen, buckwheat pollen, schisandra pollen, motherwort pollen and rape pollen) were observed by scanning electron microscope, and were characterized by surface enhanced Raman spectroscopy. The results showed that the morphological differences among pollens were small, and it was difficult to distinguish different kinds of pollens by scanning electron microscopy, while SERS could identify different kinds of pollen simply and quickly. Seven kinds of bee pollen had significant difference in the range of 1800-400 cm-1. Compared with bee pollens, Pinus massoniana Lamb pollen had more clearly discernible Raman peaks, and its main characteristic peaks appear near 1701、1657、1572、1522、1397、1293、1208、810 and 565 cm-1.