When using the TDLAS technique to measure the CO2 concentration distribution in a high-temperature combustion environment, due to the non-uniform distribution of the flow field and the weak absorption of the CO2 spectral line in the near-infrared band and easy to be interfered by the absorption of H2O, the reconstruction measurement of the two-dimensional concentration field is especially difficult. In order to solve the above problems, this paper proposes a two-dimensional concentration distribution reconstruction method of non-uniform flow field based on TDLAS technology. This methodbased on the absorption line R(50) of CO2 having the characteristic that the amplitude of R2f/1f at the center frequency approximately meet with linear superposition relationship combines the absorption information of a single spectral line with the algebraic iterative algorithm to realize the reconstruction of the CO2 concentration field of the non-uniform flow field. The simulation and experimental results show that the maximum relative error of the CO2 concentration measured by the orthogonal projection mode and the result of the flue gas analyzer is less than 13%, and the average relative error is 4.16%. The overall distribution trend is similar to the result of the flue gas analyzer.