The measurement of combustion components is significant for diagnosis of combustion. The study on measurement of carbon monoxide (CO) concentration in combustion field is realized with mid-infrared inter-band cascade lasers (ICL) at 2060 cm-1 (v=1←0,P20) to cover CO absorption line based on tunable laser absorption spectroscopy (TLAS) technology. In the experiment, the spectral line strength ratio between a pair of absorptions of water vapor (7154.35 cm-1 and 7467.77 cm-1), for H2O is the product of combustion process, is used to calculate the combustion temperature, that is used to correct spectral line strength of CO to achieve accurate measurement of CO concentration. Firstly, the TLAS temperature verification test is introduced, and the results show that the fluctuation of the test temperature is less than 45 K at each set temperature step, which indicates temperature measurement is reliable. Secondly, the calibration experiment of CO concentration measurement is carried out, and measurement error of CO concentration is under 3% compared with standard gas concentration. Finally, the experiment achieves the CO concentration at the range of 0.35‰-4.5% on methane /air flat flame furnace under different combustion conditions, with a detection limit of 0.035‰. The experiment proves the feasibility and reliability of mid-infrared absorption spectroscopy technology to measure the concentration components of combustion field, which is helpful to the study of combustion diagnosis and has great application value.