The introduction ofinsect-resistant δ-endotoxingene from Bacillus thuringiensis into Brassica napus was carried out by laser microbeampuncture. The acquisition of transgenic plants and the inheritance of transgene in T1generation were confirmed by PCR and PCR Southern blot hybridization. The bioassay oftransgenic plants showed that some of the plants exhibited tolerant to pest insects andinsect-resistance was maintained in the T1 generation. These results showed that theinsect-resistance gene was integrated into the genome of Brassica napus and was stablyinherited.