In this paper, a copper(Ⅱ) complex ［Cu(pcba)2·(phen)(H2O)］ (pcba=p-chlorbenzoic acid, phen=1,10-phenanthroline) was synthesized. The structure of the complex belongs to triclinic, P1 space group, with a=0.790 98(2) nm,b=1.072 40(4) nm, c=1.487 19(6) nm, α=100.613(3)°, β=95.239(3)°, γ=108.334(3)°, Z=2, Dc=1.638 g·cm-3, F(000)=582 unit cell parameters. The final R1=0.035 9, wR2=0.089 1. The interaction between HSA (human serum albumin) and the complex was evaluated by UV-Vis and fluorescence spectroscopy. The complex quenched the intrinsic fluorescence of HSA via static quenching with Ksv=2.35×105 L·mol-1, the quenching rate constant Kq=2.35×1013 L·mol-1·s-1, the binding constant Ka=2.14×1013 L·mol-1, the binding site n=2.37. Furthermore, the proliferation inhibition effect of the complex on the cells A549, Hela and HepG2 was studied.