Multi-layered conductive rubber was designed， aiming at the complicated process design of the filling material at the gap opening of the front shell of TFT-LCD. The metal particle filling layer with high conductivity was selected as the conductive surface layer， meanwhile the carbon cloth layer was used to realize the electromagnetic shielding function. Whereas， the flexible silicone rubber was used as the intermediate matrix layer. The synergy of low hardness and high conductivity of conductive rubber was achieved through multi-layered structure. Results showed that when the shore A hardness of silicone rubber was 30， the volume resistivity was 0.01 Ω·cm， which could meet the application requirements of sealing， waterproof and EMI shielding of TFT-LCD.