To explore the effect of Chinese herbal medicine additive on the immune function of the Macrobrachium rosenbergii, this experiment took Macrobrachium rosenbergii as an object, adding 0%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0% compound Chinese herbal additive into the feed, fed for 25 d, under the water temperature 20～30℃ and pH 7～8. The results showed that as the increase of the concentration of compound Chinese herbal medicine additive in the feed, the growth index, the activity of AKP in serum and the content of the serum protein of Macrobrachium rosenbergii showed a tendency to go up first and then down. The experiment group had greatly improvement compared with the control group. The concentration of Chinese herbal medicine increased the protein content in serum far more than the protein content in muscle did. In each experimental group, the additive amount of 2.5% had the best effect. The experiment showed that adding compound Chinese herbal medicine additive has obvious effects on immune activity and growth performance of Macrobrachium rosenbergii, which can provide basic information for healthy aquaculture of Macrobrachium rosenbergii.