Benzo(a)pyrene (Bap) is a typical organic pollutant in the environment, which is enriched in the human body through continuous biological cycles and can cause chronic damage to the organism. In order to rapidly detect benzopyrene and complete trace analysis, a new disposable sensor for the separation and detection of poisons was constructed by combining thin layer chromatography plates (TLC) with surface-enhanced Raman scattering (SERS) to detect benzopyrene. This method has high sensitivity, easy operation and other advantages. It can complete the on-site rapid analysis and detection of poisons, and effectively overcome the problems of difficult operation and high technical requirements of the current detection method for benzopyrene, which has certain practical significance.