In order to investigate the influence of micro groove arrays on the wettability of TC4(Ti6Al4V) titanium alloy surface, microgroove machining is carried out on the surface of titanium alloy using picosecond laser. Orthogonal experiments are designed and regression analysis is performed to study the relationship between laser parameters and groove size and morphology. Through single factor experiments, the effects of changes in groove width, groove depth, and groove spacing on the water contact angle of titanium alloy surfaces are studied. It is found that within a certain range, larger groove width and depth, and smaller spacing can enhance the hydrophobicity of the titanium alloy surface.