Hemoglobin (Hb) bart′s hydrops is caused by mutations or deletions affecting all four α-globin genes,leading to absent α-globin chain production from the affected genes. Laser tweezers Raman spectroscopy (LTRS) is a useful tool for single-cell analysis based on the optical trapping and Raman scattering. A LTRS setup with 785 nm laser beam was used to record the Raman scattering of trapped single erythrocytes from Hb bart′s hydrops. The results reveal that the Hb bart′s erythrocytes are possessed of not only diversiform cells and heterogeneous Raman scattering signals,but remarkable low intensity of Raman scattering and definite proportion nucleated erythrocytes,while the normal controls homogeneous. The analyse of Raman spectra and principal component analysis uncovers the Raman scattering heterogeneity of cells with normal shape and middle size. LTRS peeks insight into the abnormal biochemical essence beyond the normal cell appearance due to the heme aggregation and protein denaturalization.