Using laser-induced breakdown spectroscopy(LIBS) combined with internal standard method to achieve the aim of analyzing the information of uranium concentration in samples fast and quantitatively, the 300nm~800nm spectra of 7ns, 20mJ nanosecond laser pulse induced graphite sheet coated with uranyl nitrate were detected by echelle spectrometer and ICCD, quantitative analysis of uranium samples coated with different concentrations was done. Theoretical analysis and experimental verifications were made to obtain the spectra data of UⅡ 409.01nm, UⅡ 367.01nm, CⅠ 373.72nm, CⅡ 383.57nm and CⅢ 378.94nm. The results show that there is a good linear relationship between normalized uranium intensity and uranium concentration when the sample concentration is lower than 5.0×10-3mol/m2(surface density was studied in this paper), the combination of LIBS and internal standard method could realize the rapid quantitative analysis of uranium concentration in the samples, this study provides reference for the rapid detection and analysis of trace uranium element in nuclear contamination and uranium ore.