By the method of laser microbeam puncture, the maturing embryoes of cotton were transformed with plant bivalent expression vector pBLGC including β-1,3-glucanase and chitinase genes. Fungal challenge of T1 generation with Verticillium albo-atrum were carried out by dipping their roots into hyphal and spores solution. The survived T1 seedings were subjected to Kan-resistant assay, and the results showed that 9 out of 29 T1 plants showed obvious Kanamycin resistance (Kan 1%). Upon PCR analysis, 7 out of 9 Kan-resistant plants produced a PCR fragment with expected size, indicating that they are probably transgenic. After T1 plants in disease-inducing plot were subjected to the period, in which the Veritcillium wilt serious took place, 7 PCR positive plants showed obvious resistance to this fungus, at last, flowered and bore cotton bolls normally, while the other T1 plants and the non-transformants all died of infection. This result primarily suggested that the foreign genes had integreted in cotton genome, and the transgenic plants showed high resistance to Verticillium wilt.