The visible upconversion luminescence spectra of Ho3+ sensitized by Yb3+ were measured in different double doped (Yb3+-Ho3+) glasses under LD (0.97 μm) excitation at A.T. The fluorescence lifetimes of Yb3+ ions in single or double doped glasses were presented, and lifetimes of Yb3+ in (Yb3++Ho3+) doped glasses were smaller than those of Yb3+ doped glasses only. The upconversion luminescence of Ho3+ sensitized by Yb3+ is the most efficient, and the mechanism of the sensitization luminescence was discussed.