In order to make up for the shortcomings of the traditional liquid crystal orientation, a low-cost, simple and better orientation technology was developed. In this study, a simple experimental method was used to self-assemble ZnO nanoparticles onto indium tin oxide (ITO) glass substrate, and the nanostructure of ZnO was regulated by changing the calcination temperature. Then combined with the friction method, the preinclination of liquid crystal molecules can be adjusted. The experimental results show that the higher the calcination temperature of ZnO is, the more the preinclination of the liquid crystal molecules is close to 0°. On the contrary, the lower the calcination temperature is, the closer the liquid crystal preinclination angle is to 90°. In this study, by adjusting the self-assembled ZnO nanostructure, the preinclination of liquid crystal molecules in the range of 0°~90° was controlled.