This paper discusses multiple methods used to form ring optical trap: extracting high-order diffractive light oomponent, axioons, rotating lens and phase-only mask, compares their characteristics in terms of quasi-parallel ism of the formed ring beam, transfer performance, energy contrast between solid and vacant part of ring beam and the size of the focus after the 40× objective, and point out the rationality of using the method of extracting high-order diffractive light component. Using ring beam makes it possible to form an optical trap through a 40× objective. This is consistent with theoretical calculation.