In order to analyze experimentally the back-scattering micro-spectrum difference between human normal stomach epithetic cell and the cancerous cell to provide optical technique for clinical checking,a micro-spectral measurement setup for checking cell was established based on fiber confocal microscopically imaging technique combined with cell light scattering theory. It could provide micro-imaging and micro-medical spectral information simultaneously. Adherent monolayer human normal gastric epithelium line GES-1 and human carcinoma cell line NCI-N87 were measured with the setup respectively. The spectral results illuminated that micro-back-scattering intensity from NCI-N87 cells was stronger than that from GES-1 in 500-800 nm range,and GES-1’s scattering spectrum showed a regular intensity change but NCI-N87’s didn’t. It indicates that fiber confocal back scattering (FCBS spectrometer) micro-spectral measurement system established can distinguish normal from dyplastic cell at cellular level. The experimental results agree well with theoretical expecting results from literature. Understanding the cell scattering properties of different pathological phases will aid in the development of noninvasive optical techniques for detection of precancerous conditions.