To investigate the effect of He-Ne laser irradiation combined with antibiotic prophylaxis on postoperative incision healing after cardiac pacemaker implantation. Methods: a total of 146 patients with permanent cardiac pacemaker implantation from March 2013 to October 2018 were randomly divided into Group C (n＝73, antibiotic treatment) and group L (n＝73, He-Ne laser combined with antibiotic treatment). The clinical efficacy, postoperative incision pain, comfort, postoperative infection rate, cystic hematoma rate and incision healing time were observed and compared between the two groups. The complications such as skin peeling and burns were recorded. Results: ①There was no significant difference between the two groups in total efficiency rate and total significant efficiency (P>0.05). ②Postoperative incision pain scores in group L at 2, 3, 5 and 7 days were all lower than those of group C (P<0.05). ③After treatment, the proportion of shoulder and back pain, dysuria and poor sleep quality in group L were lower than that in group C (P<0.05). ④There were no significant difference between the two groups in hematoma, pouch infection and incision infection rate (P>0.05), but the healing time of incision in group L was significantly lower than that in group C (P<0.05). ⑤No serious skin burns, blisters or other damage occurred in group L. Conclusion: He-Ne laser irradiation combined with antibiotic prophylaxis can shorten the incision healing time after cardiac pacemaker implantation, reduce the incision pain, improve patient comfort and safety.