The residual effect of fractured glass, which is greatly affected by its fracture morphology, is correlated to the post-fracture performance of laminated glass. For tempered glass in laminated glass, its fracture morphology is affected by some major variables such as surface compressive stress, thickness, and fracture point. However, a few previous work concerning the fracture morphology characterization are reported. In this paper, fragmentation tests were firstly conducted on 81 tempered glass specimens. The fracture morphology and spatial distribution, including fragment area, perimeter, circularity and distribution intensity, were analyzed. The fracture morphology and spatial distribution obtained at the given variables (i.e., surface compressive stress of 60-105 MPa, thickness of 6-12 mm, three fracture points) were discussed. Also, a new seeds generation approach for the centroid of fragments to control nearest neighbor distance and global distribution intensity of fragments was proposed, which was combined with the Voronoi tessellation to characterize the fracture morphology of tempered glass. The proposed method has a satisfactory accuracy in generating the fracture morphology.