Stellite6 Co-based coatings were prepared through the coaxial powder-feeding laser cladding technique. The laser cladding parameters were optimized by means of the combination of orthogonal test design, single pass cladding, single-layer multi-pass cladding and multi-layer multi-pass cladding. The microstructure of the cladded coatings was characterized through scanning electron microscope, optical microscope, X-ray diffractometer, while the microhardness and the tribological performance of the coatings were also investigated. Results show that the optimal laser cladding parameters were successfully obtained through comprehensively analyzing the dilution rate, forming coefficient and microhardness of the coating. Furthermore, homogeneous microstructure was developed inside the cladded coating, which was consisted of plane crystals near the fusion zone, dendrites in the central region and equiaxed grains in the top region. The laser-cladded Stellite6 coating was composed of fcc-Co, (Co,W)3 and Cr23C6 phases, and its average microhardness reached 457 HV. The wear-resistant property of the laser-cladded Stellite6 coating was found superior compared to the 316L base metal and the wear behavior of the coating was featured of abrasive wear.