This paper proposes a new method for analyzing and characterizing green teas. The excitation-emission fluorescence matrixs (EEMs) of twenty-six types of green teas were measured with FS920 fluorescence spectrometer. The three-dimensional (3D) spectra and contour maps were obtained by measuring the emission spectra in the range from 310 nm to 750 nm, and excitation wavelengths from 300 nm to 550 nm. The excitation and emission profiles of three factors were plotted from parallel factor analysis (PARAFAC) model. The spectra characters of three main components (tea polyphenols, flavonol, chlorophyll) in green teas were ascertained. Different green teas can be characterized and distinguished with the sample projection plots of the PARAFAC model. The results demonstrate the capability of the combination of 3D fluorescence spectra technology and three-factor PARAFAC model for characterizing, differentiating and analyzing green teas.