A cross-linkable zwitterionic amphiphile was designed and synthesized, which can self-assemble into lyotropic liquid crystals for the fabrication of ordered polymeric nanostructure. Bifunctional polymerizable methacrylate groups were introduced into the amphiphilic molecule to afford structural crosslinking by radical polymerization, which can be realized by the use of 1,10-dibromodecane and diethyl malonate as starting materials in the synthesis. Then the quaternization with 1,3-propanesultone yielded the final zwitterionic product that was confirmed by nuclear magnetic resonance spectroscopy (NMR). Lyotropic liquid crystals of the molecule in water were prepared and characterized by polarizing optical microscopy (POM). Hydration of the amphiphile can form only lamellar mesophases. According to the critical packing parameter (CPP) theory, the molecular CPP of this amphiphile is between 1/2 and 1 that preferred the formation of lamellar mesophases.