The expressions of MCMs genes in hepatocellular carcinoma were analyzed based on bioinformatics. The expressions MCMs genes and proteins in HCC were significantly higher than those in normal hepatic tissue (P<0.05). The expressions of MCMs genes increased significantly with the increase of the stage of HCC (P<0.05). Expressions level of MCM2, MCM3, MCM4, MCM5, MCM6, MCM7 were significant correlation with disease-free survival (DFS) and overall survival (OS) (P<0.05). Patients with high expression of MCMs genes had poorer DFS and overall OS. We further found those proteins that interact with MCMs include MCM10, ORC1, ORC2, ORC4, ORC5, GINS1, GINS2, GINS3, GINS4, CDC6, CDC7, CDC45, ASF1B, CDT1,WDHD1. Metacape signals pathway enrichment analysis showed that MCMs genes were mainly enriched in DNA replication, cell cycle regulation, DNA-dependent DNA replication, activation of replication precursor complex, DNA uncoiling, nuclear gene replication, DNA-regulated replication, CDC6-related initial recognition complex regulation, DNA replication checkpoint regulation, protein-DNA complex assembly and other signal pathways. MCMs were highly expressed in HCC, which were significantly associated with patient prognosis. MCMs expressions showed a clear correlation. MCMs mainly interferes with DNA synthesis and replication, leading to the occurrence and development of HCC.