In order to process different microstructures on the Ti6Al4V surface and change its wettability to make the surface superhydrophobic, the nanosecond fiber pulsed laser was used to micro-fabricate the Ti6Al4V surface. The effects of the pulse energy density and the scanning interval on the lattice surface morphology and wettability of 3-D microarrays, linear arrays, and surface microstructures were investigated. The results show that the pulse energy and scanning interval affected the surface morphology parameters Sa, Sd, among which, the Sa and Sd of the rid structure were affected the most, followed by the linear array structure, while the lattice structure affected the smallest. After Ti6Al4V was processed by laser, spontaneous transition from superhydrophilic to hydrophobic or even superhydrophobic will occur on the surface. Microstructures processed with different pulse energies and scanning intervals had different degrees of surface wettability improvement, among which the grid structure had the best improvement on the surface wettability, followed by the linear array, and the worst was the lattice; The maximum and minimum contact angles of the grid, linear array, and lattice structure are 165 °, 160.5 °, 142.4 °; 132.9 °, 97 °, 94.6 °, and the surface parameters Sa and Sd with the maximum contact angle are 0.97μm, 1.38; 1.62μm, 1.04; 4.14μm, 2.39, respectively. This research has certain reference significance for improving the surface wettability of Ti6Al4V.