Comb spectrum microwave usually refers to electromagnetic wave with center frequency spectrum distribution similar to comb shape, and energy distribution at multiple frequency points within a certain bandwidth. Comb spectrum microwave has unique advantages in electronic countermeasure that other countermeasure ways do not have, and shows an excellent application prospect in communication countermeasure, radar countermeasure and other fields. This paper introduces a method to generate high power comb spectrum microwave. The microwave generated by wideband continuous wave source is compressed by using channel encoding pulse compression technology to obtain the comb spectrum microwave with center frequency of 2.85 GHz, bandwidth of 1 GHz, spectral gap of 250 kHz and peak power of 160 kW. The subsequent experiments further show that the center frequency, bandwidth and spectral gap of comb spectrum microwave obtained by this method are flexible and adjustable, which can be used in a variety of electronic countermeasure scenarios to improve the anti-jamming capability.