The incidence and mortality of liver cancer are increasing year by year. Finding effective methods to improve early screening and diagnosis of liver cancer is an important way to reduce mortality. Quantitative detection of serum alpha-fetoprotein (AFP) is an important auxiliary method for early screening and diagnosis of liver cancer. The commonly used AFP detection methods have the disadvantages of high cost, long detection time and unsuitable for large-scale population screening. The method of combining a biochip scanner based on the principle of laser confocal and a near-infrared fluorescent-labeled antibody chip is used to achieve the quantitative detection of AFP, and compare it with the commonly used Roche electrochemiluminescence immunoassay method to detect AFP content in patient samples. The results show that there is a high correlation between them.