Human eye is the key organ of three-dimensional display, it plays unique roles in perception including depth visual, space/time resolution, color/brightness response, etc. The critical characteristics of three-dimensional display such as stereo-image quality, viewing comfort, and effect of presence are investigated. The stereo visual fatigue, surface deformation, image quality of large view angle, and color ribbon effect in auto-stereoscopic display are researched. The impulse response function of lenticular lens is analyzed, and solutions are put forward. Multi-view auto-stereoscopic display is used in the science popularization of Chengjiang Biota. Unit image processing, stereo depth and resolution of integral imaging, as well as the human visual effects in holographic display are also discussed.