In view of the low frequency stabilization accuracy of prism laser gyros, the characteristics of frequency stabilization control system of prism laser gyros are systematically studied. The light intensity tuning curve and the frequency stabilizing actuator are analyzed theoretically. The mathematical model of the frequency stabilization control system of the prism laser gyros is established. The steady state performance and dynamic performance of the system are further analyzed. The analysis results show that the system has different steady-state errors and the adjustment time is too long under the influence of constant temperature and slow temperature change, which leads to the decline of the stability frequency accuracy. We optimize the controller parameters to provide optimal damping ratio and rapidity for second-order system. And the feedforward control system with temperature compensation is adopted to realize the full compensation of the frequency stability error caused by the temperature and improve the frequency stability accuracy of the system. The experimental results show that the optimized frequency stabilization control system can improve the frequency stability accuracy by one order of magnitude compared with the original frequency stabilization control system, and the gyro accuracy can be improved by more than 30%.