To explore the effects of riboflavin on renal fibrosis in IL-17A-/- mice induced by fluoride, 32 wild-type and 32 IL-17A-/- 6-week-old C57BL/6J male mice were randomly divided into the control group (0.9% NaCl), sodium fluoride group (24 mg/kg NaF), riboflavin group (5 mg/kg riboflavin sodium phosphate), and sodium fluoride plus riboflavin group (24 mg/kg NaF + 5?mg/kg riboflavin sodium phosphate), 8 mice each group. Mice were treated by gavage for consecutive 90 days, then the renal morphological changes, fibrosis degree, and serum levels of Cr, UA, and BUN were detected. The results showed that riboflavin could reverse the degree of renal histological damage and fibrosis in mice caused by fluoride. Riboflavin did not alleviate the increase of serum Cr, UA and BUN levels induced by fluoride in wild type mice. The results suggested that riboflavin could alleviate the renal fibrosis degree induced by fluoride in wild type and IL-17A-/- mice, and IL-17A-/- mice presented better mitigation effect.