The study on the influence of hardening and swelling courses on gelatin hologram during the post-processing procedure is conducted experimentally. The result showed that a hardened gelatin film with diluted F-5 fixing bath, can make lower the required exposure for the maximum diffraction efficiency of holograms. And the swelling level in water is very important to diffraction efficiency, a method to gain good swelling results is presented in this paper, in which the swelling temperature is determined by the ultimate swelling temperature.