In order to identify an excellent gene source that can be used for soybean genetic improvement, a soybean GmWIN1-6 transcription factor was obtained from soybean (Glycine max) genome identification. Using bioinformatics tools and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) technology, the physical and chemical properties, protein structure, temporal and spatial expression profile and salt stress of GmWIN1-6 transcription factor were systematically analyzed. The results showed that the open reading frame (ORF) of GmWIN1-6 gene was cloned from soybean flower tissue, its encoded protein consists of 176 amino acids, has a conserved AP2 domain at the N-terminus, and is a hydrophilic protein. In the secondary structure of GmWIN1-6 protein, random coils account for the largest proportion. The tertiary structure is similar to AtWIN1 of Arabidopsis (Arabidopsis thaniana). Phylogenetic analysis showed that GmWIN1-6 is closely related to AtWIN1. The expression of GmWIN1-6 transcription factors in soybean tissues is significantly different. Among them, the expression level is the highest in flower tissues, followed by higher expression levels in the middle and late stages of seed development, and basically coincides with the accumulation period of seed oil. Under salt stress in soybean seedlings, GmWIN1-6 gene up-regulated expression. These experimental findings indicate that the GmWIN1-6 transcription factor may be involved in the regulation of soybean seed development and oil accumulation and seedling stress response, and provide a theoretical basis for soybean genetic improvement and molecular breeding.