To investigate the effects of oridonin on migration and invasion of human chondrosarcoma cells. In vitro experiments, human chondrosarcoma SW-1353 cells were divided into control group being without intervention, 1.25, 2.50, 5.00μmol/L oridonin groups (1.25, 2.50, 5.00 μmol/L oridonin) and positive drug group (6 μmol/L adriamycin). Human chondrosarcoma SW-1353 cells were tested for viability, mobility, invasion ability and epithelial-mesenchymal transition (EMT) expression. The results show that: compared with the control group, SW-1353 cell viability in the 5.00 μmol/L oridonin group andpositive drug group significantly decreased (P<0.05). 1.25, 2.50, 5.00 μmol/L oridonin groups and positive drug obviously inhibted cell migration and invasion in a concentration-dependent manner (P<0.05). The expression of E-cadherin decreased theexpression of vimentin and N-cadherin and inhibited EMT process by increasing the expression of E-cadherin protein, and thehigher the concentration, the more significant the change was (P<0.05). Lamonidin can significantly inhibit the migration andinvasion of human chondrosarcoma SW-1353 cells, or through mediating EMT, which provides a theoretical basis for the biological study of human chondrosarcoma cells.