The damage threshold of an interline transfer CCD irradiated by different wavelength nanosecond Raman lasers was studied and an experiment with 496 nm, 574 nm, 630 nm Raman and multispectral Raman laser-irradiated CCD was carried out. The damage threshold interval of dot damage, line damage and total damage were observed and collected by adjusting the energy of each focused Raman laser. By careful fitting, the damage threshold interval and the damage possibility curve of the CCD at different laser energy densities with each Raman laser were estimated. Results showed that the multispectral Raman laser including a residual pump laser is most effective for damaging the CCD than the monochrome Raman laser, and the 630nm Raman laser acts better than 574 nm and the 496 nm Raman laser. The microscopic images of the damaged CCD were reviewed, and the electronic characters of the damaged CCD were also tested to understand the damage and blindness mechanism of a Raman laser pulse-irradiated CCD.