A novel fiber Bragg grating with micro-structured defect is proposed and demonstrated. Its temperature characteristics are studied experimentally. It was obtained by wet chemical etching part of fiber Bragg grating in different concentrations of hydrofluoric acid solutions. The change of the local effective refractive index in the etched area induces two resonant peaks. Experimental results show that the two resonant peaks have different temperature sensitive coefficients. The wavelengths of the two resonant peaks become 0.8127 nm and 0.7329 nm longer while the temperature increases from 20 ℃ to 90 ℃, and the temperature sensitive coefficients are 0.01161 nm/℃ and 0.01047 nm/℃ respectively. The fitting linearity is 0.991 and 0.998 respectively. So the two resonant peaks of novel fiber grating present good linear relationship with temperature.