Journal of Innovative Optical Health Sciences, Volume. 15, Issue 4, 2250024(2022)

Supercontinuum fiber laser-based coherent anti-Stokes Raman scattering microscopy for label-free chemical imaging

[in Chinese]1,2, [in Chinese]1,2, [in Chinese]1,2、*, [in Chinese]1,2, [in Chinese]3, [in Chinese]1,2, [in Chinese]1,2, [in Chinese]3, [in Chinese]1,2, and [in Chinese]1,2
Author Affiliations
  • 1Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, P. R. China
  • 2Engineering Research Center of Molecular & Neuro Imaging of the Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, P. R. China
  • 3Shaanxi Eye Hospital, Xi'an People's Hospital (Xi'an Fourth Hospital), Affliated Guangren Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, P. R. China
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    Coherent anti-Stokes Raman scattering (CARS) microscopy can resolve the chemical components and distribution of living biological systems in a label-free manner and is favored in several disciplines. Current CARS microscopes typically use bulky, high-performance solid-state lasers, which are expensive and sensitive to environmental changes. With their relatively low cost and environmental sensitivity, supercontinuum fiber (SF) lasers with a small footprint have found increasing use in biomedical applications. Upon these features, in this paper, we homebuilt a lowcost CARS microscope based on a SF laser module (scCARS microscope). This SF laser module is specially customized by adding a time-synchronized seed source channel to the SF laser to form a dual-channel output laser. The performance of the scCARS microscope is evaluated with dimethyl sulfoxide, whose results confirm a spatial resolution of better than 500 nm and a detection sensitivity of millimolar concentrations. The dual-color imaging capability is further demonstrated by imaging different species of mixed microspheres. We finally explore the potential of our scCARS microscope by mapping lipid droplets in different cancer cells and corneal stromal lenses.

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    [in Chinese], [in Chinese], [in Chinese], [in Chinese], [in Chinese], [in Chinese], [in Chinese], [in Chinese], [in Chinese], [in Chinese]. Supercontinuum fiber laser-based coherent anti-Stokes Raman scattering microscopy for label-free chemical imaging[J]. Journal of Innovative Optical Health Sciences, 2022, 15(4): 2250024

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    Paper Information

    Received: Feb. 18, 2022

    Accepted: Mar. 13, 2022

    Published Online: Aug. 27, 2022

    The Author Email: (wtzhou@xidian.edu.cn)

    DOI:10.1142/s1793545822500249

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