The process of thermal denaturation of the albumin was studied using dynamic speckle method based on wavelet entropy. At first, the dynamic speckle pattern sequences generated by albumin colloid during denaturing were acquired using charge-compled device (CCD) camera. Using the speckle sequences, the time history of speckle patterns (THSP) were generated. And then, with the wavelet entropy as a parameter, each row of THSP was separated into eight time windows which were decomposed by db4 orthogonal wavelet family into three levels . Thus a three-dimensional matrix of 256×256×8 of wavelet entropy was generated. Finally, this matrix was grayed into eight 256×256 patterns, which made the change of dynamic speckle signals visulized. According to the patterns, the movement properties of the protein molecule ensemble were analyzed during thermal denaturation of the albumin. The results show that this method is effective to analyze the process of movement and aggregation of protein molecules quantitatively. Experimental results prove that this method is a useful tool to investigate the motion of particles in solution.